Nucleotide Pyrophosphatase Activity in Yeast Extracts
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چکیده
منابع مشابه
Purification and properties of a yeast nucleotide pyrophosphatase.
A nucleotide pyrophosphatase (EC 3.6.1.9) has been purified from extracts of the hybrid yeast, Saccharomyces fragilis X Saccharomyces dobzhanskii, to an extent where it appears homogeneous by ultracentrifugation and sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Multiple protein components seen on polyacrylamide and starch gel electrophoresis also exhibit enzyme activity. Ultracentr...
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Enzymatic splitting of diphosphopyridine nucleotide (DPN)’ by microorganisms, plants, and animal tissues has been known for many years, but the precise nature of this reaction is not well understood (1). Ohlmeyer (2) first observed that adenylic acid (5-phosphoadenosine) appeared when DPN was incubated with yeast maceration juice. Later Heiwinkel (3) identified adenylic acid as a product of the...
متن کاملYeast Inorganic Pyrophosphatase
The kinetics of the Mg2+ activation of crystalline yeast inorganic pyrophosphatase have been thoroughly investigated from pH 7.40 to pH 9.05 using a sensitive isotope assay in order to determine the role of the divalent metal ion activator in the reaction. A computer program has been devised for calculating the concentration of each of the various components of the complex equilibrium involving...
متن کاملYeast Inorganic Pyrophosphatase
The magnesium ion-dependent hydrolysis of pyrophosphate catalyzed by yeast inorganic pyrophosphatase is markedly inhibited by calcium ion. The kinetics of inhibition have been measured and analyzed in terms of a model involving strong interactions between CaPPi and two metalcomplexed forms of the enzyme, EMg and ECa. Inhibition constants for these interactions have been estimated by nonlinear r...
متن کاملEnzymatic Activity Assays in Yeast Cell Extracts.
Saccharomyces cerevisiae (S. cerevisiae) (commonly known as baker's yeast) is a model organism that has a similar upstream base excision repair (BER) pathway for the repair of methylated bases as that in mammalian cells, and it is very easy to maintain in the laboratory environment. Here, we described a method to prepare cell extracts from yeast to investigate their enzymatic activities. This p...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1961
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)64323-6